We explore a method in which MSC condensations can be formed reliably utilizing a Schiff base reaction between modified polysaccharides. Polysaccharides are naturally occurring molecules that can be slightly modified to promote their crosslinking and interaction.
Cells are first suspended in a polysaccharide (PS 1) containing free primary amine groups. They are subsequently pipetted into droplets of a second polysaccharide featuring aldehyde groups (PS 2) that crosslink spontaneously with the amines of PS 1. The resulting microtissues can be used as tools to explore cell behaviors such as stem cell differentiation, or they can be applied to tissue defects where they serve to regenerate tissue.
More information can be found at: http://onlinelibrary.wiley.com/doi/10.1002/adhm.201500102/abstract